Anti-AMPylated Tyrosine

Analysis Note

ControlRac1, AMPylated in vitro

Application

Research CategorySignaling

Research Sub CategoryGeneral Post-translation Modification

Anti-AMPylated Tyrosine Antibody detects level of AMPylated Tyrosine & has been published & validated for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

AMPylation is a reversible post-translational modification in which adenosine monophosphate (AMP) derived from ATP is added to threonine and tyrosine (and possibly serine) residues on target proteins. This modification is created by proteins containing Fic (filamentation induced by cyclic adenosine monophosphate) domains, which are present in both prokaryotic and eukaryotic cells. In mammals, the HYPE protein is the most widely known Fic-containing protein and the precise function of its AMPylated targets is not yet clear. However, bacterial cells possess numerous Fic-domain proteins that modify and disable protein targets in host cells, thereby suppressing signaling pathways and increasing the robustness of pathogenic infections. The Vibrio parahaemolyticus VopS protein, for instance, disrupts the function of Rho guanine triphosphatases, and eventually disrupts the actin cytoskeleton.

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blot in Rac1, AMPylated in vitro.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected AMPylated Tyrosine on 10 ng of Rac1, AMPylated in vitro.

Specificity

Broad species reactivity expected, as AMPylation is evolutionarily conserved.

This antibody recognizes AMPylated Tyrosines.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~21 kDa observed. Molecular weights will vary based on the protein being AMPylated.